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. 2020 Sep 25;11:4869. doi: 10.1038/s41467-020-18693-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a parg-1 mutants display SPO-11-dependent accumulation of RAD-51 foci. Error bars represent S.E.M. Asterisks indicate statistical significance assessed by unpaired t test (***=p < 0.0001 and “ns” indicates not significant). Analysis was performed in at least three gonads for each genotype. Number of nuclei analysed for each genotype (from zone 1 to 7 respectively): WT (200, 205, 127, 181, 162, 170, 90); parg-1(gk120) (191, 256, 248, 177, 158, 126, 135); spo-11 (242, 236, 221, 204, 159, 158, 96); spo-11 parg-1(gk120) (205, 218, 203, 188, 135, 105, 81). b Representative examples of cells at different stages of the same genotypes analysed in (a). Scale bar 5 μm. c Analysis of diakinesis nuclei in different genotypes before and 27 h after exposure to IR. Error bars represent standard deviation. Center of error bars indicates mean. Statistical analysis was performed with two-tailed nonparametric Mann–Whitney test. (***p < 0.0001 and ns indicates not significant differences). Number of nuclei analysed at 0 and 10 Gy respectively: WT (35–94), parg-1(gk120) (33–88), com-1 (37–55), com-1; parg-1 (42–48), mre-11 (124–52), parg-1; mre-11 (110–40). d Representative images of diakinesis nuclei of the same genotypes scored in (c). Scale bar 5 μm.