Table 1.
Gene comparison between the Rickettsia asembonensis complete genome from this study and those previously detected in fleas, ticks and in blood samples across the world.
| Source | Vertebrate host | Location, Country | Year1 | gltA | 17 kDa | ompB | ompA | Reference | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Identities2 | GenBank acc. no | Identities2 | GenBank acc. no | Identities2 | GenBank acc. no | Identities2 | GenBank acc. no | |||||
| Ctenocephalides felis |
Human (Homo sapiens) Grizzly (Ursus arctosi) |
Columbia, SC, USA | 2009 | 371/372 (99.7%) | AY953289 | 432/434 (99.5%) | AY953286 | Nelder et al.40 | ||||
| C. felis | Opossum (Didelphis virginiana) | Orange Co., CA, USA | 2016 | 1035/1035 (100%) | KU597068 | 1517/1517 (100%) | KU597066 | 1224/1225 (99.9%) | KU597069 | Krueger et al.15 | ||
| C. felis | Cat (Felis catus) | Los Angeles Co., CA, USA | 2016 | 787/787 (100%) | KP398499 | Billeter et al.41 | ||||||
| C. felis | Cat (Felis catus) | Georgia, USA | 2016 | 613/613 (100%) | KX431974 | 833/833 (100%) | KX431985 | Šlapeta and Šlapeta12 | ||||
| C. felis | Opossum (Didelphis virginiana) | Orange Co., CA, USA | 2016 | 787/787 (100%) | KP3984994 | Maina et al.29 | ||||||
| C. felis | Cat (Felis catus) | Galveston, TX, USA | 2019 | 342/342 (100%) | MH325379 | 394/394 (100%) | MH325383 | 769/770 (99.9%) | MH325368 | Blanton et al.4 | ||
| C. felis | Dog (Canis lupus) | Coclé province, Panama | 2011 | 724/724 (100%) | HM582437 | Bermudez et al.42 | ||||||
| Pulex simulans | Dog (Canis lupus) | Costa Rica | 2016 | 350/350 (100%) | KJ5690905 | Troyo et al.43 | ||||||
| Amblyomma ovale | Dog (Canis lupus) | Costa Rica | 2016 | 349/349 (100%) | KX544811 | 559/559 (100%) | KX544817 | Troyo et al.43 | ||||
|
Rhipicephalus microplus |
Cow (Bos taurus) | Costa Rica | 2016 | 350/350 (100%) | KJ5690905 | Troyo et al.43 | ||||||
| C. felis | Collected from a bed | Villeta, Colombia | 2016 | 350/350 (100%) | KJ569090 | 382/382 (100%) | KJ569091 | Faccini-Martínez et al.44 | ||||
| C. felis | Dog (Canis lupus) | Imperatriz, Brazil | 2017 | 1018/1018 (100%) | KY445726 | 528/528 (100%) | KY445730 | 794/794 (100%) | KY445737 | Silva et al.17 | ||
| C. felis | Dog (Canis lupus) | Edison Lobão, Brazil | 2017 | 1109/1110 (99.9%) | KY445725 | 532/532 (100%) | KY445735 | 762/762 (100%) | KY445740 | Silva et al.17 | ||
| Rhipicephalus sanguineus | Dog (Canis lupus) | Tapes, Brazil | 2017 | 1136/1137 (99.9%) | KX196267 | 369/369 (100%) | KX196268 | Dallagnol et al.45 | ||||
| C. felis VGD7 | NA | Iquitos, Peru | 2018 | 1314/1314 (100%) | KY650697 | 476/476 (100%) | KY650697 | 4944/4947 (99.9%) | KY650699 | 4159/4164 (99.9%) | KY650698 | Loyola et al.16 |
|
Ctenocephalides canis 8294D3 C. felis LER205 |
NA NA |
Iquitos, Peru Puerto Maldonado, Peru |
2019 |
1314/1314 (100%) 1314/1314 (100%) |
476/476 (100%) 476/476 (100%) |
4947/4947 (100%) 4947/4947 (100%) |
5151/5151 (100%) 5151/5151(100%) |
Loyola et al.16 | ||||
| Blood | Human (Homo sapiens) | Various localities, Peru | 2018 | 382/383 (99.7) | LN831076 | Palacios-Salvatierra et al.19 | ||||||
| Archaeopsylla erinacei |
Hedgehog (Erinaceus europaeus) |
Munich, Germany | 2009 | 335/335 (100%) | EU927696 | 770/770 (100%) | EU927697 | Gilles et al.46 | ||||
| Pulex irritans | Dog (Canis lupus) | Hungary | 2010 | 341/341 (100%) | EU853838 | Hornok et al.47 | ||||||
| Echidnophaga gallinacea | Black rats Rattus rattus) | Egypt | 2006 | 341/341 (100%) | DQ166938 | 394/394 (100%) | DQ166937 | Loftis et al.48 | ||||
| Synosternus pallidus | Cat (Felis catus), Dog (Canis lupus, traps in homes | Sine-Saloum, Senegal | 2012 | 726/728 (99.7%) | JF966774 | 770/770 (100%) | JF966775 | Roucher et al.49 | ||||
| C. canis | Dog (Canis lupus) | Nyanza, Kenya | 2013 | 1127/1130 (99.7%) | JN315968 | 371/371 (100%) | JN315968 | 819/820 (99.8%) | JN315972 | 1515/1517 (99.9%) | JN315977 | Jiang et al.50 |
| C. felis | Dog (Canis lupus) | Nyanza, Kenya | 2013 | 1311/1314 (99.8%) | JWSW01000078 |
501/501 (100%) |
JWSW01000044 |
4944/4947 (99.9%) |
JWSW01000087 | 5080/5088(99.8%) | JWSW01000076 | Jima et al.18 |
| C. felis | Dog (Canis lupus) | Musanze, Rwanda | 2018 | 611/612 (99.8%) | MH142453 | 831/833 (99.7%) | MH142452 | Nziza eet al.51 | ||||
| C. felis | Dog (Canis lupus) | Mazabuka, Zambia | 2019 | 320/321 (99.7%) | LC431490 | 696/696 (100%) | LC431491 | 208/208 (100%) | LC431502 | Moonga et al.52 | ||
| C. canis | Dog (Canis lupus) | Sangkhlaburi, Thailand | 2003 | 1166/1171 (99.6%) | AF516333 | 772/774 (99.7%) | JX183538 | Parola et al.53 | ||||
| C. felis | Dog (Canis lupus) | Bangkok, Thailand | 2011 | 328/328 (100%) | JF511463 | 390/390 (100%) | JF511461 | Foongladda et al.54 | ||||
| C. felis | Dog (Canis lupus) | Bangkok, Thailand | 2018 | 132/134 (98.5%) | MG264737 | 304/305 (99.7) | MG452137 | Mongkol et al.55 | ||||
| C. felis | Dog (Canis lupus) | Nan province, Thailand | 2019 | 525/525 (100%) | MK660561 | Takhampunya et al.56 | ||||||
| Blood | Cat (Felis catus) | Bangkok, Thailand | 2018 | 1160/1168 (99.3%) | MH523411 | Phoosangwalthong et al.22 | ||||||
| C. felis |
Dog (Canis lupus) Cat (Felis catus) |
Kuala Lumpur, Malaysia Pulau Pinang, Malaysia | 2014 | 725/725 (100%) | KF963606 | Tay et al.57 | ||||||
| Ctenocephalides orientis | Dog (Canis lupus) | Peninsular Malaysia | 2017 | 1136/1137 (99.9%) | KX1962673 | Low et al.58 | ||||||
| Rhipicephalus sanguineus | Dog (Canis lupus) | Peninsular Malaysia | 2017 | 748/749 (99.9%) | MF281711 | Low et al.58 | ||||||
| Blood |
Long-tailed macaque (Macaca fascicularis) |
Peninsular Malaysia | 2015 | 210/210 (100%) | KP126803 | 777/779 (99.7%) | KP126804 | Tay et al.21,22 | ||||
| Blood | Human (H. sapiens) | Kuala Lumpur, Malaysia | 2016 | 399/402 (99.2%) | KU255716 | 816/818 (99.8%) | KU255717 | Kho et al.20 | ||||
| Ceratophyllus fasciatus | Rat (Rattus rattus) | Himachal Pradesh, India | 2015 | 341/341 (100%) | HM370112 | 792/794 (99.7%) | HM370113 | Cahota et al.59 | ||||
| C. orientis | Dog (Canis lupus) | Delhi, Mumbai, Rajasthan, India | 2015 | 586/586 (100%) | KP256357 | 794/795 (99.9%) | KP256359 | Hii et al.60 | ||||
| Xenopsylla ramesis | Wild rodents | Negev, Israel | 2015 | 340/340 (100%) | KP050777 | 332/332 (100%) | KP050778 | 739/740 (99.9%) | KP050780 | 505/505 (100%) | KP050779 | Rzotkiewicz et al.61 |
Identical and almost identical (> 99.9%) matches are in bold.
The sca4 gene was omitted from the table to reduce its size. This gene was sequenced in only 4 studies15,16,18,50,61, which produced sequences with 100% identity in relation to the R. asembonensis detected in this study.
NA = Information not available, C. felis = Ctenocephalides felis, C. canis = Ctenocephalides canis, C. orientis = Ctenocephalides orientis.
1Refers to the year of publication.
2 refers to the number of identical bp in the comparison, the total number of overlapping bp and the similarity percentage (in parenthesis).
3While Low et al. 2017 did not create a GenBank entry for their gltA sequences they stated the genotype they found was a 100% match to acc. number KX196267.
4While Maina et al. 2016 did not create a GenBank entry for their ompB sequences they stated the ompB genotype they found was a 100% match to acc. number KP398499.
5While Troyo et al. 2016 did not create a GenBank entry for some of their gltA sequences they stated that the genotype they found was a 100% match to the acc. number KJ569090.