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. 2020 Sep 24;11(9):798. doi: 10.1038/s41419-020-02921-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a WB of PIK3R3 and p21 both in LoVo and SW48 cells transfected with siNC, siPIK3R3#1, and siPIK3R3#2. b CCK8 assays of LoVo and SW48 cells transfected with PIK3R3-specific siRNA. c BrdU-positive cells percent of PIK3R3-knockdown LoVo and SW48 cells. d Cell cycle distribution of LoVo and SW48 cells transfected with siNC, siPIK3R3#1, and siPIK3R3#2 was measured by flow cytometry. e, f SA-β-gal staining of PIK3R3-knockdown LoVo or SW48 cells after treatment of Dox (0.25 μM, 48 h). Every assay repeated three times, and these numeral results were displayed as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, t-test.