Fig. 5.

PTPN14–SOCS7 complex regulates the expression of inflammatory factors. a, b PTPN14-deficient BMDMs were transfected with Socs7 siRNA and were stimulated with 1 μg/mL LPS and 5 mM D-GalN for 24 h. qRT-PCR (a) and ELISA (b) were used to detect the expression levels of TNF-α, IL-1β, IL-12, and IL-18. qRT-PCR data (a) were representative of one experiment with at least three independent biological replicates; a single data point represented one technical repeat. Data shown in b were cumulated from three independent experiments (mean ± s.e.m. of n = 3). Two-tailed Student’s t-test was used to compare the means between the two groups. **p < 0.01.