Fig. 3.

MAC5A interacts with SE. (A) The interactions of MAC5A with various proteins detected by BiFC. The MAC5A-CDC5 interaction is used as a positive control. Paired cCFP- and nVenus-fusion proteins were transiently coexpressed in N. benthamiana leaves. Green color indicates the BiFC signal (originally yellow fluorescence) detected by confocal microscopy at 48 h after infiltration. One hundred nuclei were examined for each pair and a picture is shown. Red: autofluorescence of chlorophyll. (B and C) Co-IP analyses of the MAC5A-CDC5 (B), and the MAC5A-SE interactions (C). CDC5-GFP or MAC5A-GFP/GFP were coexpressed with MYC-MAC5A or MYC-SE in N. benthamiana, respectively. IP was performed using anti-GFP antibodies. MYC-MYC5A, MYC-SE, CDC5-GFP, MYC5A-GFP, and GFP were detected by immunoblot. (D) The interaction between MAC5A and SE detected by Co-IP in Arabidopsis. Total proteins were extracted in transgenic plants harboring p35S::GFP or gMAC5A-GFP. IP was performed using anti-SE antibody. After IP, GFP and MAC5A-GFP proteins were detected by immunoblot using anti-GFP antibody. Inputs in B–D show the total protein before IP. RNase A was used to digest RNA stands.