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. 2020 Sep 2;117(38):23617–23625. doi: 10.1073/pnas.2008980117

Fig. 3.

Fig. 3.

UBXD18 regulates the sensitivity of Dictyostelium to decanoic acid. (A) A UBXD18 knockout (UBXD18−) was generated by inserting a blasticidin resistance sequence (BsR) into the ubxd18 gene, and a rescue (UBXD18−/+) was generated by expressing the ubxd18 gene attached to GFP. (B) Growth analysis of wild-type (n = 12), UBXD18− (n = 12), and UBXD18−/+ (n = 9) Dictyostelium cells in decanoic acid, displayed as dose–response curves of normalized cell density plotted against log concentration. (C) IC50 values were compared between wild type (n = 12), UBXD18− (n = 12), and UBXD18−/+ (n = 9) (one-way ANOVA with Dunnett’s post hoc test). (D) Analysis of p-4E-BP1 levels in wild type, UBXD18−, and UBXD18−/+, with MCCC1 as a loading control (n = 8) (one-way ANOVA with Dunnett’s post hoc test). (E and F) Analysis of p-4E-BP1 levels in (E) UBXD18− (n = 9) (Kruskal–Wallis test with Dunn’s post hoc test) or (F) UBXD18−/+ (n = 9) (one-way ANOVA with Dunnett’s post hoc test) treated with decanoic acid (60 µM) for 1 or 24 h, with MCCC1 as a loading control. Data represent the mean ± SEM. Significance is indicated by *P ≤ 0.05, ***P ≤ 0.001, ****P ≤ 0.0001.