Table 1.
No. mutations | HpC* | κ* | Buffer | Rg (Å) | ν | χ2r | |
PNt | – | 0.0300 | 0.222 | Native | 51.10 ± 0.13 | 0.542 ± 0.002 | 1.095 |
Gdn | 58.78 ± 0.11 | 0.583 ± 0.001 | 0.982 | ||||
Swap1 | 6 (1.8%) | 0.0458 | 0.212 | Native | 49.20 ± 0.59 | 0.514 ± 0.009 | 0.904 |
Gdn | 59.87 ± 0.20 | 0.580 ± 0.002 | 0.966 | ||||
Swap2 | 18 (5.4%) | 0.0697 | 0.218 | n.d. | n.d. | n.d. | |
Swap3 | 18 (5.4%) | 0.0445 | 0.200 | Native | 40.58 ± 1.07 | 0.432 ± 0.017 | 0.909 |
Gdn | 61.26 ± 0.64 | 0.594 ± 0.006 | 1.023 | ||||
Swap4 | 21 (6.3%) | 0.0329 | 0.170 | Native | 53.37 ± 0.17 | 0.558 ± 0.002 | 1.069 |
Gdn | 60.19 ± 0.34 | 0.584 ± 0.003 | 0.975 | ||||
Swap4.1 | 21 (6.3%) | 0.0296 | 0.213 | Native | 54.45 ± 0.14 | 0.545 ± 0.002 | 0.938 |
Gdn | 58.97 ± 0.23 | 0.580 ± 0.002 | 1.033 | ||||
Swap5 | 12 (3.6%) | 0.0302 | 0.291 | Native | 48.71 ± 0.34 | 0.498 ± 0.005 | 0.924 |
Gdn | 59.95 ± 0.38 | 0.584 ± 0.004 | 1.015 | ||||
Swap6 | 12 (3.6%) | 0.0694 | 0.137 | Native | 52.61 ± 0.27 | 0.562 ± 0.003 | 1.067 |
Gdn | 59.25 ± 0.50 | 0.583 ± 0.005 | 0.934 |
See Materials and Methods for explanations of the HpC and κ calculations. n.d., not determined.