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. 2020 Sep 2;117(38):23774–23781. doi: 10.1073/pnas.2004129117

Fig. 4.

Fig. 4.

Complementation of S44S through restoration of mRNA in a proximal site. (A) The UTR of the endogenous locus was mutated at AA111CT from the TSS so that 5′-CU-3′ would interact with 5′-AG-3′, thereby stabilizing the disrupted hly structure. (B) Quantification of LLO secretion by Western blot. (C) Infection of CD-1 mice (i.v. dose = 105) for 48 h. (D) Phagosomal escape assay using colocalization of bacteria to P62 in the presence of cytochalasin D. Error bars represent SDs from three independent replicates. Significance was determined by unpaired t test for B and C and paired t test for D. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, NS = not significant (P > 0.05).