Fig. 1.

BLACAT1 promotes cell proliferation, migration and invasion, yet dampens cell apoptosis in OS. BLACAT expression level was measured by qRT-PCR analysis in OS cell lines (143B, U2OS, MG63, Saos2) versus normal osteoblast hFOB1.19. B. The inhibitory efficiency of sh-BLACAT1#1/2 was detected by qRT-PCR analysis. C-D. Cell proliferation ability was evaluated by EdU and colony formation assays after transfecting sh-BLACAT1 #1/2 into selected OS cells. E. Apoptotic cells were evaluated by TUNEL assay after silencing BLACAT1. F-G. Wound-healing and transwell invasion assays were performed to assess cell migration and invasion ability after silencing BLACAT1. H. western blot was conducted to measure the level of proteins related to cell apoptosis and migration. *P < 0.05; **P < 0.01.