Fig. 2.

BLACAT1 sponges miR-608 in OS. Nucleus-cytoplasm separation assay was used to determine the subcellular distribution of BLACAT1 in 143B and MG63 cells. B. RegRNA and miRDB bioinformatics tools predicted the candidate miRNAs for BLACAT1. C. Candidate miRNA expressions were measured by qRT-PCR in OS cell lines and normal hFOB1.19 cells. D. RNA pull down was conducted to testify the physical combination between BLACAT1 and miR-608. E. RIP assay showed the abundance of BLACAT1 and miR-608 in antibody targeting Ago2. F. Putative miR-608 binding site in BLACAT1 predicted by. G. Luciferase reporter assays was performed in 143B and MG63 to verify the interaction between BLACAT1 and miR-608. **P < 0.01.