Fig. 3.

SOX12 is the target of miR-608. A. MiRTarBase and miRDB databases predicted potential targets of miR-608. B. QRT-PCR analysis was conducted to study the expression of above mRNA candidates after transfecting miR-608 mimics into 143B and MG63 cells. C. The expression pattern of SOX12 was detected in OS cells versus normal controls by qRT-PCR. D. RNA pull down assay verified the binding relationship between SOX12 and miR-608 in 143B and MG63 cells. E. Luciferase reporter assays were conducted to determine the relationship between SOX12 and miR-608. F-G. QRT-PCR and western blot analyses were utilized to study the expression of SOX12 mRNA and protein after co-transfecting miR-608 inhibitor into BLACAT1-silenced 143B and MG63 cells. **P < 0.01.