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Schematic representation of the genetic organization of the genomic islands (GIs), integrative conjugative element (ICE), and prophages and location of reporter genes in the tagged genome. Selectable (cat), counter-selectable (sacB), and chromogenic (lacZ) alleles were introduced in the V. cholerae genome, adopting an allelic exchange method. Asterisk indicates that Vibrio pathogenicity island-1 (VPI-1) was tagged with sacB-cat as well as sacB-cat-lacZ alleles.
