Fig. 3.

Genetic organization of chromosomal integration sites (attB) before and after excision of all of four genomic islands from the genome of N16961. (A) attL and attR sites were formed due to site-specific recombination between genomic island attachment site (attP) and chromosomal attachment site (attB). Lane numbers 17 and 18 contained 1-kb and 100-bp DNA ladders, respectively. (B) Polymerase chain reaction (PCR) analysis of N16961 and its derivatives in the presence and absence of genomic islands (GIs). Amplified PCR products were resolved in an agarose gel. Lane number and corresponding amplicon are mentioned in A. Blue arrows indicate primers that bind to the upstream and downstream of GI, and green arrows indicate primers that bind to the GI.