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Genetic tools engineered to measure the integration efficiency of different GIs, ICE (SXT), and CTXΦ. The integration module of all of the elements was amplified from their excised circular form, which include attachment site (attP) with or without tyrosine recombinases. The original vector (pSW23T) was unable to replicate in V. cholerae due to its conditional ori (R6K) function.
