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. 2020 Sep 1;117(38):23762–23773. doi: 10.1073/pnas.2006283117

Table 1.

Relevant genotype and phenotype of wild-type and genetically modified Vibrio cholerae strains used in the study

Strain Genotype and phenotype Reference
N16961 Wild type, O1 El Tor, Stpr 13
O395 Wild type, O1 classical, Stpr 5
VCE232 Wild type, O4 environmental 5
41081 O1 El Tor, SXT+ve, NAr, Funr, Plmr MVIDH, India
MO10 Wild type, O139 serogroup, Stpr, SXT+ 22
BS1 N16961ΔTLCΦ-CTXΦ-dif1::lacZ-dif1Δdif2, Stpr, Spcr 25
BS11 BS1ΔrecA, Stpr, Spcr 25
AP7 N16961ΔrecA, Stpr This study
AP8 VCE232ΔrecA::aph, Kanr This study
AP10 O395ΔrecA::aph, Stpr, Kanr This study
SB23 N16961vsp1::sacB-cat, Stpr, Camr, Sucs This study
SB25 N16961Δvsp1, Stpr This study
DD1 N16961vsp2::sacB-cat, Stpr, Camr This study
DD2 N16961Δvsp2, Stpr This study
SB30 N16961vpi1::sacB-cat, Stpr, Camr, Sucs This study
SB31 N16961Δvpi1, Stpr This study
SB26 N16961vpi2::sacB-cat, Stpr, Camr, Sucs This study
SB27 N16961Δvpi2, Stpr This study
SB32 N16961Δvsp1 vsp2::sacB-cat, Stpr, Camr, Sucs This study
SB33 N16961Δvsp1 Δvsp2, Stpr This study
SB42 N16961Δvpi2, ∆lacZ, Stpr This study
SB43 N16961Δvpi1, ∆lacZ, Stpr This study
SB44 N16961Δvsp1 Δvsp2lacZ, Stpr This study
SB51 N16961Δvpi2lacZvpi-1::sacB-cat-lacZ, Stpr, Camr, Sucs This study
SB52 N16961 ∆vpi-1 ∆vpi-2 ∆lacZ, Stpr This study
SB45 N16961Δvsp1Δvsp2lacZvpi1::sacB-cat-lacZ, Stpr, Camr, Sucs This study
SB47 N16961Δvsp1Δvsp2 ∆vpi1 ∆lacZ, Stpr This study
SB46 N16961Δvsp1Δvsp2lacZvpi-2::sacB-cat-lacZ, Stpr, Camr, Sucs This study
SB48 N16961 ∆vsp1 ∆vsp2vpi-2 ∆lacZ, Stpr This study
SB49 N16961Δvsp1Δvsp2∆vpi1∆lacZvpi2::sacB-cat-lacZ, Stpr, Camr, Sucs This study
SB50 N16961Δvsp1Δvsp2 ∆vpi1∆vpi2 ∆lacZ, Stpr This study
SB53 N16961Δvpi1Δvpi2Δvsp1Δvsp2ΔlacZΔTLCΦ-RS1-CTXΦ-dif1::aadA, Stpr, Specr This study
SB58 N16961Δvsp1::pBD60, Stpr, Camr This study
SB59 N16961Δvsp2:: pBD60, Stpr, Camr This study
SB56 N16961::cat, Stpr, Camr This study
SB60 N16961 Δvsp1 Δvsp2::cat, Stpr, Camr This study
SB62 O395::sh ble, Stpr, Zeor This study
SB63 N16961 ∆vpi-1 ∆vpi-2::pBD60, Stpr, Camr This study
BB1 N16961Δvpi1Δvpi2Δvsp1Δvsp2ΔlacZΔTLCΦ-RS1-CTXΦ-dif1::lacZ-dif1 This study
BB2 N16961Δvpi1Δvpi2Δvsp1Δvsp2ΔlacZΔTLCΦ-RS1-CTXΦ-dif1::lacZ-dif1- CTXΦ-sacB-cat This study
BS20 BS1ΔlexA, Stpr, Spcr, Kanr 26
BD14 BS20 lexA(Ind ) 26
JV2 41081SXT:ΔstrAB, Stpr NAr, Funr, Plmr This study
JV3 41081SXT:ΔstrAB::sacB-aadA, Stpr NAr, Funr, Plmr, Specr This study
JV4 41081ΔSXT, Stpr, NAr, Funr, Plmr This study
JV5 BS1ΔlexAΔrecA, Stpr, Spcr, Kanr This study