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. 2020 May 13;69(8):1779–1792. doi: 10.2337/db19-0829

Figure 3.

Figure 3

MT-overexpressing BM-MNC transplantation improves blood perfusion and ischemic angiogenesis in db/db mice with HLI. BM-MNCs from WT and JTMT mice were transplanted in db/db mice immediately after HLI; MNC suspension buffer was used as vehicle control. The blood perfusion was monitored before and at 0, 3, 7, 14, 21, and 28 days after HLI using a PeriCam PSI (A) and quantified by Image J (B). Ischemic hind limb muscle tissues were then collected at day 28 after HLI. Transverse sections of gastrocnemius (GS) muscle (C) and soleus (SS) muscle (D) were stained by CD31 to evaluate angiogenesis. Capillary density was expressed as CD31+ capillaries per high-power field (HPF) (E), and DAPI was used to recognize nuclei. Data are mean ± SD. n ≥ 8 mice/group. *P < 0.05 vs. vehicle; #P < 0.05 vs. WT.