Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 13;69(8):1779–1792. doi: 10.2337/db19-0829

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the American Diabetes Association

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. More information is available at https://www.diabetesjournals.org/content/license.

PMC Copyright notice

Knockdown of HIF-1α abolishes MT overexpression, protecting EPCs against HG and hypoxia-induced EPC dysfunction. BM-EPCs from WT and JTMT mice were transfected with the specific siRNA against HIF-1α for 48 h; the Silencer Select Negative Control was used as control siRNA (Ctrl-siRNA). Then the transfected EPCs were exposed to HG (25 mmol/L) and hypoxia for an additional 24 h, the equivalent concentration of mannitol was used as osmotic control (Ctrl). The angiogenic function was evaluated by tube formation assay (A and B). The migration capability was evaluated by TEM assay (C and D). Three independent experiments were performed. Data are mean ± SD. *P < 0.05 vs. Ctrl-siRNA-Ctrl; #P < 0.05 vs. Ctrl-siRNA-HG; $P < 0.05 vs. WT-Ctrl-siRNA-HG.