Fig. 6.

TDP-43 overexpression induces blood-brain barrier permeability. a Representative images depicting CD3+ T cells in the frontal cortex of mice. Scale bar = 10 μm. b Manual count of CD3+ T cells. Statistical analysis was carried out using one-way ANOVA with Bonferroni post hoc test (n = 4 mice/group, ***p < 0.001). c Representative images depicting CD4+ T cells in the frontal cortex of mice. Scale bar = 10 μm. d Manual count of CD4+ T cells. Statistical analysis was carried out using one-way ANOVA with Bonferroni post hoc test (n = 4 mice/group; ***p < 0.001). e Representative images depicting mouse IgG in the frontal cortex of mouse tissue. Scale bar = 20 μm. f Quantification of percent area of the frontal cortex covered by mouse IgG staining. Statistical analysis was carried out using a one-way ANOVA with Bonferroni post hoc test (n = 4 mice/group, ***p < 0.001). g Representative western blots of frontal cortex lysate probed for mouse IgG, ICAM1, VCAM1, caveolin 1, and actin. h Quantification of mouse IgG, ICAM1, VCAM, and caveolin 1 levels normalized to actin. Statistical analysis was carried out using a one-way ANOVA with Bonferroni post hoc test (n = 4 mice/group, *p < 0.05). i Representative images of Prussian blue staining depicting microhemorrhages in the frontal cortex of mice. Scale bar = 20 μm. j Quantification of percent area of the frontal cortex covered by Prussian blue-stained deposits. Statistical analysis was carried out using a one-way ANOVA with Bonferroni post hoc test (n = 4 mice/group, *p < 0.05)