Fig. 3.

Reporter gene assays revealed a concentration-dependent interaction of miR-142-5p on MCL1 3′-UTR. MCL1 3′-UTR containing vectors were co-transfected with pre-miR-142-5p in two concentrations (10 nM, 25 nM). After 48 h, reporter gene activities were measured. a Normalized reporter gene activity on MCL1 3′-UTR (WT) was repressed by miR-142-5p 16% [10 nM] and 23% [25 nM]. b Mutagenesis of the predicted seed region 2230–2233 bp (MUT 1) abolished this inhibitory effect. c The prediction of miR-142-5p binding to MCL1 3′-UTR are illustrated including mutated bases highlighted in gray. All reporter gene activities (n ≥ 12 in 3 independent experiments) were normalized to activities of cells transfected with respective 3′-UTR target sequence vectors and pre-miR negative control (median ± interquartile range). Activities are shown relative to empty control vector (c) identically transfected and normalized as 3′-UTR target sequence vectors. Mann–Whitney U-test; ***p ≤ 0.001