Fig. 5.

Reporter gene assays showed an interaction of miR-142-5p with SRI 3′-UTR. Co-transfection of the vectors containing the 3′-UTR of SRI with pre-miR-142-5p was performed in two concentrations (10 nM and 25 nM). 48 h after transfection, reporter gene activities were measured. a MiR-142-5p repressed reporter gene activity of wild type SRI 3′-UTR (WT) by 26% [10 nM] and 8% [25 nM]. b Predicted interaction of miR-142-5p with SRI 3′-UTR. All activities (n ≥ 12 in 3 independent experiments) were normalized to activities of cells transfected with respective 3′-UTR target sequence vectors and pre-miR negative control (median ± interquartile range). Activities are shown relative to empty control vector (c) identically transfected and normalized as 3′-UTR target sequence vectors; Mann–Whitney U-test; **p ≤ 0.01, *p ≤ 0.05