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. 2020 Sep 25;10:15780. doi: 10.1038/s41598-020-72804-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Structure confirmation of 2-methyl-pentanoyl-carnitine. (a) Extracted ion chromatograms of co-injection experiment of the synthetic 2-MPC spiked to a positive plasma extract (blue) in comparison to the positive plasma extract (black). (b) MS/MS fragmentation comparison of the synthetic and natural molecules (collision-induced dissociation at 20 V). (c) IR spectra generated with infrared ion spectroscopy (IRIS) analysis on the protonated ion at m/z 260 from the HPLC purified marker (black), 2-MPC (red) and 3-MPC (blue). (d) Structure of 2-MPC.