Fig. 2. Other paradigms of DR also require p38-MAPK pathway components.

a The life span of eat-2(ad465) is suppressed by sek-1 RNAi. b The increased life span of eat-2(ad465) is partially abrogated in eat-2(ad465);sek-1(km4). The above life spans were performed concurrently. c In bacterial dilution-induced DR (BDR), decreasing concentration of bacterial food produces a bell-shaped curve in WT. However, in sek-1(km4), no life-span extension is observed at any of the dilutions. Bacterial culture of OD₆₀₀ = 3.0 was the starting concentration for serial dilution. The increase in life span when WT was maintained at OD₆₀₀ = 1.0, 0.5, 0.25, or 0.125 was significant as compared to OD₆₀₀ = 3.0 (purple stars); the difference in case of sek-1(km4) was not. Two-way ANOVA-Tukey’s multiple comparisons test. ***P = 0.0005, ****P ≤ 0.0001. Also, the average life spans of WT at dilutions OD₆₀₀ = 1.0, 0.5, 0.25, and 0.125 were significantly different from sek-1(km4) (dark blue stars). Two-way ANOVA-Sidak multiple comparisons test. *P = 0.0472, **P = 0.0015 (for OD₆₀₀ 0.25), **P = 0.0021 (for OD₆₀₀ 0.125), ***P = 0.0005. n = 4 independent experiments. Data are presented as mean values +/− SEM. d The life span of WT as well as sek-1(km4) is extended to the same extent when the worms were grown on 2-deoxyglucose (2-DOG). Life span was performed at 20 °C and life-span summary data is provided in Supplementary Table 1.