Fig. 3. Metabolic reprogramming associated with DR does not require p38-MAPK.

a Knocking down drl-1 produces a DR-like phenotype in both WT as well as sek-1(km4), resulting in depletion of stored fat to similar extent. Quantified data is provided in Supplementary Fig. 2a. One of three independent experiments is shown. b Depletion of stored fat during DR, as seen in eat-2(ad465), is independent of sek-1. The sek-1 RNAi did not significantly change the low fat of eat-2(ad465). Quantified data is provided in Supplementary Fig. 2b. One of three independent experiments is shown. Multiple overlapping images were acquired at ×100 magnification to cover the entire worm body and stitched together to generate a contiguous image. c Knockdown of drl-1 decreased ROS levels to similar extent in WT and sek-1(km4), as determined by DCFDA assay. Data are presented as mean values ± SEM. n = 4 independent experiments. Two-way ANOVA-Sidak’s multiple comparisons test, ***P = 0.0004, **P = 0.0050. d Oxygen consumption rate (OCR) measured using Oroboros O2K shows similar respiratory rates in WT and sek-1(km4). Data are presented as mean values ± SEM. n = 4 independent experiments. Unpaired two-tailed t test, **P = 0.0011, ***P = 0.0006. e Autophagy, as determined by puncta formation in the seam cells of a LGG-1::GFP-expressing strain (upper panel), was increased in both WT as well as in sek-1(km4) when drl-1 was knocked down by RNAi. Representative images (upper panel) and quantification (lower panel) from one of two independent experiments shown. Experiments performed at 20 °C. n = 18 worms. Data are presented as mean values ± SEM. Two-way ANOVA-Sidak’s multiple comparisons test, ****P ≤ 0.0001. Scale bar = 10 μm. Experiments were performed at 20 °C. Source data are provided as a Source data file.