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. 2020 Sep 25;11:4865. doi: 10.1038/s41467-020-18690-4

Fig. 4. CyTP genes are upregulated during DR-like condition in a p38-MAPK-dependent manner.

Fig. 4

a Quantitative reverse transcriptase PCR (QRT-PCR) analysis shows that many Cytoprotective genes (CyTP) genes, of the GO category “xenobiotic detoxification pathway” (XDP), that are upregulated when drl-1 is knocked down in WT, but failed to do so to the same extent in sek-1(km4). Expression levels are normalized to actin. n = 2 biologically independent samples. Data are presented as mean values ± std. dev. b The expression of Pcyp-35B1::gfp is enhanced at YA stage when drl-1 is knocked down using RNAi, compared to control RNAi. However, the upregulation is abrogated if sek-1 is also knocked down by RNAi. Representative images from one of three independent experiments shown. Multiple overlapping images were acquired at ×100 magnification to cover the entire worm body and stitched together to generate a contiguous image. Experiments performed at 20 °C. Source data are provided as a Source data file.