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. 2020 Sep 25;10:15750. doi: 10.1038/s41598-020-72837-3

Figure 2.

Figure 2

Example Western blots of the HLA antibodies used for expression analysis of HLA-G and HLA-F. (A) HLA-G1 and -G3 were assessed using anti-HLA-G antibody clone 4H84 [cropped blots images of HLA-G1 and -G3 as well as GAPDH after stripping are obtained from the same blot; see supplemental figure HLA-G1 (4H84)]. Soluble HLA-G isoforms HLA-G5 and -G6 were assessed using anti-HLA-G antibody clone 5A6G7 [cropped blots images of HLA-G5 and -G6 as well as GAPDH after stripping are obtained from the same blot; see supplemental figure HLA-G (5A6G7)]. (B) HLA-F1 expression was measured using an anti-HLA-F antibody Aviva [cropped blots images of HLA-F1 as well as GAPDH after stripping are obtained from the same blot; see supplemental figure HLA-F (Aviva)]. HLA-F3 expression was assessed using an anti-HLA-F antibody from Sigma Aldrich [cropped blots images of HLA-F3 as well as GAPDH after stripping are obtained from the same blot; see supplemental figure HLA-F (Sigma)]. HER2 human epidermal growth factor receptor 2, HLA human leukocyte antigen, kDa kilodalton.