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. 2020 Sep 25;10:15804. doi: 10.1038/s41598-020-72503-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Rab13 regulates and co-localizes with sEV markers. (A) Cell lysates prepared from DKO-1 cells expressing an empty shRNA vector (E), a scrambled shRNA vector (S), or shRNAs targeting Rab13. Western blots were performed with antibodies against β1-integrin, CD63, CD81, and TSG101. (B) Quantification of immunoblots (n = 3) of sEV markers under Rab13 knockdown conditions. (C) Immunolocalization of Rab13, sEV and plasma membrane (plasma membrane Ca2+ ATPase, PMCA) markers in DKO-1 cells. Cells were stained with DAPI (blue), Rab13 (magenta), or antibodies against β1-integrin, CD63, CD81, or PMCA (green). Rab13 co-localized with the plasma membrane when cell–cell contact was made between neighboring cells. Significance was analyzed by one way ANOVA. **p < 0.01, ***p < 0.001. Data represent mean ± SE, n = 3.