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. 2020 Sep 25;10:15804. doi: 10.1038/s41598-020-72503-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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β1-integrin+/Rab13+ EVs are distinct from CD63+ exosomes. Direct immunoaffinity capture (DIC) was used to purify vesicles associated with either β1-integrin or CD63⁸. (A) Vesicles from the P10 centrifugation step were immunoaffinity captured with antibodies against β1-integrin and western blots were performed on the captured material using antibodies against β1-integrin, CD63 or Rab13. (B) Vesicles from the P100 centrifugation step were immunoaffinity captured with antibodies against either CD63 or β1-integrin and western blots were performed as in A. DIC shows a distinction between β1-integrin + sEVs and CD63+ classical exosomes, with an enrichment of Rab13 in the β1-integrin + sEVs.