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. 2020 Sep 25;10:15754. doi: 10.1038/s41598-020-72838-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Images of teeth with intact proximal surface (score 0) and hidden dentin caries (score 3). (a) Occlusal view of proximal contacts. (b) Histological view of the area of interest stained with 5% acid red solution. Left: Enamel demineralization penetrating the entire enamel thickness. However, breakdown of enamel surface was not evident (white allow). Dentin caries with slight cavitation was observed penetrating along DEJ (red arrow, score 3). Right: Intact (score 0). (c) Intraoral digital radiograph. Evidence of caries was not clear (white arrow). (d) En face intensity image generated by 3D OCT. (e) Extracted 2D image of the area along the red line in d. Left: Brightness of proximal zone was significantly increased penetrating the entire enamel thickness (white arrow). The brightness of the DEJ was increased because of lateral spread of the lesion along DEJ (red arrow). Because of the slight cavitation along DEJ, intensity of brightness at DEJ appears higher than Fig. 3e left. Right: Brightness of proximal surface was not increased and almost constant. The dynamic slicing 3D video is presented in Supplementary Materials: Video 3.