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. 2020 Sep 25;11:4871. doi: 10.1038/s41467-020-18715-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The wild-type TadA and the evolved TadA monomer for ABEmax are light blue and orange stadiums, respectively, while the miniABEmaxV82G is depicted as a yellow stadium. b On-target activity profile of different ABE permutants tested. nSpCas9-variants of ABE permutants were challenged against the YFP and ABE16 loci. Data are pooled from three independent technical replicates, showing the mean (colored bar) activity at several adenine positions measured for adenine-to-guanine editing. c Heatmap showing the off-target profile of different permutations of nSpCas9 ABEs. Off-target events were considered at the position of the most heavily deaminated adenine in the promiscuous DNAJB, SCAP, MTA2, SAP30BP, LCMT1, and PTPB2 transcripts (three independent technical replicates of three biological replicates targeting YFP, ABE16, and non-targeting were included). Adenosine (A) to inosine (I) editing efficiencies are shown as the mean value scaled from the lowest to highest value across all samples for all RNA off-target sites. Source data are available in the Source data file.