Figure 3.

Gating pore current is small in Na_V1.4-R219K and undetectable in Ca_V1.1-R897K. (A) Gating pore current was observed for test potentials < −80 mV (n = 5, respectively). Leak currents recorded were subtracted by the linear fitted line from −20 mV to +20 mV in each oocyte. Error bars indicate standard error of the mean. No gating pore current was detected for oocytes expression Ca_V1.1-R897K in two different conditions: (B) external Na⁺ or (C) a mixture of 60 mM N-methylglucamine and 60 mM guanidine. For (B), n = 8 oocytes, and for (C), n = 6 oocytes.