(A and B) Wild-type (WT) and ΔmlaF strains were infected intranasally into mice. At 36 h after infection, lungs (A) and spleens (B) were harvested, and bacterial burdens were enumerated (n = 9–10 with median; significance by Mann-Whitney test).
(C) WT and ΔmlaF strain survival in Infasurf pulmonary surfactant after 90 min (n = 6 from two independent experiments; data are means ± SEM; significance by t test).
(D) Distribution of DPPC (m/z 734.5719) by imaging mass spectrometry. Scale bar is 4 mm.
(E) Activation of HEK-Blue TLR4 reporter cell line at multiplicity of infection 10−2 (n = 3; data are means ± SEM; significance by one-way ANOVA). *p < 0.05, ***p < 0.001, ****p < 0.0001. See also Figure S2.