Table 1.
The ΔmlaF Mutant Has Increased Antibiotic Susceptibility That Is Largely Restored in the Suppressor Strain
| Antibiotic | WTa | ΔmlaFa | ΔmlaF suppa | WT suppa |
|---|---|---|---|---|
| Chloramphenicol | 12 ± 3 | 12 ± 1 | 14 ± 3 | 12 ± 1 |
| Tetracycline | 20 ± 1 | 18 ± 1 | 18 ± 1 | 19 ± 1 |
| Streptomycin | 13 ± 1 | 14 ± 0 | 14 ± 1 | 14 ± 1 |
| Gentamicin | 20 ± 2 | 28 ± 2** | 21 ± 1 | 21 ± 3 |
| Erythromycin | 21 ± 1 | 27 ± 2* | 21 ± 1 | 20 ± 4 |
| Colistin | 12 ± 0 | 13 ± 1 | 11 ± 1 | 11 ± 0 |
| Polymyxin B | 8 ± 0 | 9 ± 0 | 8 ± 0 | 9 ± 1 |
| Daptomycin | 6 ± 1 | 6 ± 0 | 6 ± 1 | 7 ± 1 |
| Carbenicillin | 20 ± 4 | 33 ± 3*** | 19 ± 1 | 23 ± 2 |
| Meropenem | 19 ± 5 | 37 ± 3*** | 18 ± 2 | 24 ± 4 |
| Bacitracin | 10 ± 0 | 16 ± 1**** | 17 ± 1**** | 9 ± 0 |
| Copper chloride | 8 ± 1 | 9 ± 1 | 9 ± 0 | 9 ± 0 |
| Acetic acid | 9 ± 0 | 8 ± 0 | 9 ± 1 | 9 ± 0 |
| Hydrochlorous acid | 8 ± 0 | 8 ± 0 | 8 ± 0 | 8 ± 1 |
| Hydrogen peroxide | 12 ± 1 | 13 ± 1 | 13 ± 1 | 12 ± 1 |
| Paraquat | 10 ± 4 | 18 ± 2* | 13 ± 1 | 12 ± 3 |
Antibiotic susceptibility was determined by a disk-diffusion assay measuring the zone of clearing. Data are two or three replicates and representative of at least two independent experiments. Significance is by one-way ANOVA with Dunnett’s multiple comparisons; significant differences compared to wild-type (WT) are indicated by * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
a
Mean diameter ± standard deviation (mm); limit of detection, 6 mm.