KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and Virus Strains | ||
| A. baumannii 17978 (wild-type; WT) | ATCC | Skaar 13-89 |
| A. baumannii 17978 ΔmlaF(A1S_3103)::Kn | This manuscript | Skaar 47-65 |
| A. baumannii ABUW AB5075 | (Gallagher et al., 2015; Jacobs et al., 2014) | Skaar 56-43 |
| A. baumannii ABUW AB5075 mlaF136::T26(Tc) | (Gallagher et al., 2015) | Skaar 56-44 |
| A. baumannii ABUW AB5075 mlaF188::T26(Tc) | (Gallagher et al., 2015) | Skaar 56-45 |
| A. baumannii ABUW AB5075 mlaF160::T26(Tc) | (Gallagher et al., 2015) | Skaar 56-46 |
| A. baumannii 17978 pWH1266 | This manuscript | Skaar 39-16 |
| A. baumannii 17978 pLDP9 | This manuscript | Skaar 39-17 |
| A. baumannii 17978 ΔmlaF::Kn pWH1266 | This manuscript | Skaar 39-18 |
| A. baumannii 17978 ΔmlaF::Kn pLDP9 | This manuscript | Skaar 39-19 |
| A. baumannii 17978 pLDP29 | This manuscript | Skaar 46-42 |
| A. baumannii 17978 ΔmlaF::Kn pLDP29 | This manuscript | Skaar 46-43 |
| A. baumannii 17978 pLDP35 | This manuscript | Skaar 56-41 |
| A. baumannii 17978 ΔmlaF::Kn pLDP35 | This manuscript | Skaar 46-44 |
| A. baumannii 17978 pLDP28 | This manuscript | Skaar 45-49 |
| A. baumannii 17978 ΔmlaF::Kn pLDP28 | This manuscript | Skaar 45-50 |
| A. baumannii 17978 ΔmlaF::Kn ACX60_03855::ISAba11 (suppressor 1) | This manuscript | Skaar 47-66 |
| A. baumannii 17978 ΔmlaF::Kn ACX60_03855::ISAba11 (suppressor 2) | This manuscript | Skaar 47-67 |
| A. baumannii 17978 ΔmlaF::Kn ACX60_03855::ISAba11 (reconstructed suppressor) | This manuscript | Skaar 56-40 |
| A. baumannii 17978 ΔmlaF::Kn att::Tn7(lacI-Plac-ispB) ΔispB::Tc | This manuscript | Skaar 56-39 |
| A. baumannii 17978 ACX60_03855::ISAba11 (reconstructed suppressor in WT background) | This manuscript | Skaar 56-42 |
| A. baumannii 17978 pLDP53 | This manuscript | Skaar 59-09 |
| A. baumannii 17978 ΔmlaF::Kn ACX60_03855::ISAba11 pLDP29 | This manuscript | Skaar 59-10 |
| A. baumannii 17978 ΔmlaF::Kn ACX60_03855::ISAba11 pLDP53 | This manuscript | Skaar 59-11 |
| A. baumannii 17978 ΔmlaF::Kn pLDP52 | This manuscript | Skaar 59-14 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| FM4-64 | ThermoFisher (Waltham, MA) | T13320 |
| Critical Commercial Assays | ||
| MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel - 32 Plex | EMD Millipore (Burlington, MA) | MCYTMAG70PMX32BK |
| Pro-Q Emerald 300 Lipopolysaccharide Gel Stain Kit | Molecular Probes/Invitrogen (Carlsbad, CA) | P20495 |
| BacLight Membrane Potential Kit | Invitrogen (Carlsbad, CA) | B34950 |
| Deposited Data | ||
| Whole genome sequencing of Skaar 47-65, Skaar 47-66, and Skaar 47-67 A. baumannii strains | This manuscript | NCBI SRA BioProject: PRJNA656143 |
| Experimental Models: Cell Lines | ||
| HEK-Blue hTLR4 | Invivogen (San Diego, CA) | hkb-htlr4 |
| Experimental Models: Organisms/Strains | ||
| C57BL/6J | Jackson Laboratory (Bar Harbor, ME) | # 664 |
| B6.B10ScN-Tlr4lps-del/JthJ | Jackson Laboratory (Bar Harbor, ME) | # 7227 |
| Oligonucleotides | ||
| Listed in Table S3 | ||
| Recombinant DNA | ||
| CarbR; allelic exchange vector with sucrose resistance | (Hoang et al., 1998) | pFLP2 |
| CarbR; contains a non-polar kanamycin insertion | (Ménard et al., 1993) | pUC18-K1 |
| KnR; mobilization helper plasmid | (Figurski and Helinski, 1979) | pRK2013 |
| CarbR; helper plasmid encoding the site-specific TnsABCD Tn7 transposition pathway | (Kumar et al., 2010) | pTNS2 |
| mini-Tn7-AmpR on a suicide vector containing the R6Kγ-ori | (Carruthers et al., 2013) | pKNOCK-mTn7-Amp |
| Acinetobacter – E. coli shuttle vector | (Hunger et al., 1990) | pWH1266 |
| Contains an FRT-flanked kanamycin resistance gene | (Datsenko and Wanner, 2000) | pKD4 |
| pMMB67EH with RecAb system | (Tucker et al., 2014) | pAT02 |
| Contains regions upstream and downstream of mlaF to create pLDP8 | This manuscript | pCRBlunt-3103::Km |
| pFLP2-ΔmlaF::Kn | This manuscript | pLDP8 |
| pWH1266-PmlaF-mlaF | This manuscript | pLDP9 |
| pWH1266-PrpsA-pldA | This manuscript | pLDP28 |
| pWH1266-PrpsA | This manuscript | pLDP29 |
| pWH1266-PrpsA-mlaF | This manuscript | pLDP35 |
| pWH1266-PrpsA-ispB | This manuscript | pLDP53 |
| Contains an FRT-flanked tetracycline resistance gene from A. baumannii AYE | This manuscript | pLDP55 |
| pFLP2-ΔispB::Tc | This manuscript | pLDP56 |
| pFLP2-suppIS (upstream of ispB containing ISAba11) | This manuscript | pLDP70 |
| pKNOCK-mTn7-Amp-lacI-Plac-ispB | This manuscript | pLDP77 |
| Software and Algorithms | ||
| Prism 7 | GraphPad (La Jolla, CA) | N/A |
| Canvas X 16 | Canvas GFX | N/A |
| Breseq | (Deatherage and Barrick, 2014) | N/A |
| ISFinder | (Siguier et al., 2006) | https://www-is.biotoul.fr |
| Bowtie2 | (Langmead and Salzberg, 2012) | http://bowtie-bio.sourceforge.net/bowtie2/index.shtml |
| Samtools | (Li et al., 2009) | http://samtools.sourceforge.net/ |
| Integrated Genomics Viewer (IGV) | (Robinson et al., 2011) | http://software.broadinstitute.org/software/igv/ |
| phiSITE Promoter Hunter | (Klucar et al., 2010) | http://www.phisite.org/ |
| ImageJ Fiji | (Schindelin et al., 2012) | https://imagej.net/Welcome |
| Other | ||
| Curosurf (poractant alfa) | Chiesi (Cary, NC) | NDC 10122-510-01 |
| Infasurf (calfactant) | ONY Inc. (Amherst, NY) | N/A |