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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Neurobiol Learn Mem. 2019 Jul 18;164:107042. doi: 10.1016/j.nlm.2019.107042

Figure 1. Impaired tone recall in Fmr1 KO mice after fear conditioning.

Figure 1.

A) Schematic of training protocol: On day 1, mice were habituated to Context A and Context B for 10 min each. 24 hours later mice were trained with 5 tone-shock parings in Context A and returned to their home cage. Recall of the tone and context memories were tested 24 hours later with 3½ hours between tests. 30 min after the final recall session brain tissue was harvested. B) Habituation: All animals had low levels of freezing during habituation. C) During training both genotypes increased their freezing significantly across the training session from Tone 1 to Tone 5; however FrC Fmr1 KO mice consistently froze at a lower level than FrC WT mice. D) 24-hour Recall: Fmr1 KO mice had impaired freezing during tone recall and contextual +tone. (Individual data for each test is shown in E, F and G). H) FrC WT mice froze more than Nv WT on all three recall tests; I) but FrC Fmr1 KO mice increased freezing to context and context + tone recall only. Asterisks indicate results from t-test (A-B) or paired comparison from a two-way (C) or three-way (D-I) RM ANOVA. (*, **, *** / p = 0.05, .01, 0.001). N: WT Nv = 24; WT FrC = 32; Fmr1 KO Nv = 22; Fmr1 KO FrC 26.