Figure 5.
Overexpression of SLC9A1 blocked the functions of adMSC-Exos in DDP sensitivity of BC cells. (A) mRNA expression in cells after SLC9A1-OE administration measured by RT-qPCR; (B) cells were exposed to an ascending series of DDP, and then the IC50 value of DDP in cells was determined using a CTG kit; then, cells were treated with different exosomes and exposed to 5 μmol/mL or 10 μmol/mL DDP, respectively; (C) viability of cells determined by EdU-labeling assay; (D) cell proliferation measured by colony formation assay; (E) apoptosis of cells measured by flow cytometry; (F) LDH secretion in cells measured by a LDH kit; (G) caspase-3 activity in cells measured by a caspase-3 kit. Data were presented as mean ± SD. In all panels, data were compared by one-way ANOVA and Tukey’s multiple comparison test. *p < 0.05, ***p < 0.001 vs Exo + Vector NC. Repetition = 3.