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. 2020 Sep 26;11(9):810. doi: 10.1038/s41419-020-03011-w

Fig. 3. Knockdown of RNF115 impairs the fusion of autophagosome with lysosome.

Fig. 3

a, b HeLa cells were transfected with shRNA or shRNF115 for 24 h, then with mTagRFP-GFP-LC3 for another 24 h and EBSS treatment for 2 h before confocal microscopy analysis. The quantification of GFP+RFP+ puncta/cell was calculated. Data are means ± s.d. of at least 50 cells scored. c, d GFP-LC3 stably expressing HeLa cells were cultured on confocal dishes and transfected with shcontrol or shRNF115 for 48 h and EBSS treatment for 2 h. Then cells were stained with anti-LAMP1, and observed by confocal microscopy. The number of GFP-LC3 puncta/cell colocalized with LAMP1 was calculated. Data are means ± s.d. of at least 50 cells scored. Scale bar, 25 μm. **p < 0.01; ***p < 0.001.