Fig. 5. RNF115 knockdown in BGC823 cells inhibits cell growth, reduces tumorigenicity and metastasis.
a BGC823 cells stable infected with pLVX-shcontrol or pLVX-shRNF115 were seeded in 96-well plates (3 × 103 cells/well; five replicates), serum starved for 18 h and then pulsed with 10% FBS for indicated time. Cell viability was detected. Data are means ± s.d. of results from three independent experiments. b Representative images of colony formation by indicated cells. c Number of clones counted in three independent experiments. d, e BGC823 cells stable infected with pLVX-shcontrol or pLVX-shRNF115 (4 × 106 cells/mouse) were injected subcutaneously in BALB/c nude mice. At day 19, excised xenograft tumors were imaged and weighed (n = 6). f BGC823 cells stable infected with pLVX-shcontrol or pLVX-shRNF115 (6 × 106 cells/mouse) were injected into the abdominal cavity of mouse. At day 28, the mice were sacrificed and the metastatic nodules was observed and photographed. Arrows indicate the disseminated tumor nodules. *p < 0.05; **p < 0.01.