Fig. 3.

Caprin1 is a direct target of miR-199a-5p in CRC cells. a Predicted miR-199a-5p binding sites within the 3′-UTR of Caprin1 mRNA. b The mRNA level of Caprin1 was significantly increased in CRC tissues and adjacent normal tissues (n = 50). c Caprin1 protein levels were was significantly increased in CRC cell lines HT29, SW620, SW480, HCT116, compared with that in NCM460 cells. d The negative correlation between Caprin1 and miR-199a-5p levels in CRC tissues (r =  − 0.7750, P < 0.01). e The SW480 and HTC116 cells were transfected with miR-199a-5p mimic/inhibitor or corresponding NC, and the Caprin1 protein level was measured using western blot analysis. f The HTC116 cells co-transfected with pGL3cM-Caprin1-3ʹ-UTR or pGL3cM-Caprin1-mut-3ʹ-UTR, miR-199a-5p mimic/inhibitor or corresponding NC for 48 h, and the relative luciferase activity were measured. β-actin was used as an internal control