Fig. 5.

Restoration of Caprin1 reverses the suppressive effects of miR-199a-5p on cell growth. The HTC116 or SW480 cells were transfected with miR-199a-5p mimics or mimics NC, or were co-transfected with miR-199a-5p mimics and pcDNA-Caprin1 for 48 h. a The transfection efficiency of pcDNA-Caprin1 was identified in HTC116 or SW480 cells transfected with pcDNA-Caprin1 or pcDNA-vector by western blot. b Caprin1 protein expression was detected using western blot. c and d The reduction of cell proliferation caused by miR-199a-5p mimics was reversed by upregulation of Caprin1. e and f The increased activity of caspase 3 induced by miR-199a-5p mimics was also attenuated by upregulation of Caprin1 in HTC116 and SW480 cells (**P < 0.01 vs. mimics NC; ^##P < 0.01 vs. miR-199a-5p mimics)