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. 2020 Sep 22;12:8773–8785. doi: 10.2147/CMAR.S260537

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© 2020 Liu et al.

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PVT1 is a sponge of miR-106b-5p and negatively regulates miR-106b-5p expression in CRC cells. (A) The potential binding sites between PVT1 and miR-106b-5p were shown. (B and C) Luciferase reporter assay was used to demonstrate the relationship between PVT1 and miR-106b-5p in HCT116 and HT29 cells, respectively. (D) The relative level of miR-106b-5p was measured using qRT-PCR in HCT116 and HT29 cells transfected with sh-NC, sh-PVT1, vector or PVT1 vector. (E) The correlation between miR-106b-5p and PVT1 was analyzed using Pearson’s correlation analysis. *P<0.05.