Figure 5.
MiR-106b-5p directly targets FJX1 and suppresses FJX1 expression. (A) The potential binding site between FJX1 and miR-106b-5p were exhibited. (B and C) Luciferase reporter assay was performed to demonstrate the relationship between FJX1 and miR-106b-5p in HCT116 and HT29 cells, respectively. (D–F) The relative level of FJX1 mRNA and protein were measured using qRT-PCR or Western blot assay in HCT116 and HT29 cells transfected with miR-NC, miR-106b-5p, anti-miR-NC or anti-miR-106b-5p. (G) The correlation between miR-106b-5p and FJX1 expression was analyzed using Pearson’s correlation analysis. *P<0.05.