FIGURE 1.

Denoising program and parameter evaluations of mock data. The number of sequences observed that are “exact,” “partial,” and “miss” matches for mock community sequences are shown for each denoising pipeline (vertical facets) and filtering parameters (horizontal facets). Values beneath each group reflect the number of ASVs combined among all four mock replicates in that group. “Exact” matches reflect 100% alignment identity between ASV detected in mock community and a known mock sequence, “partial” reflects between 97% and 99.9% identity, and “miss” represents an ASV with <97% identity to known mock sequences