FIGURE 4.

miR‐212‐5p down‐regulates SIRT2 by targeting the 3′‐UTR of SIRT2 mRNA. A, Schematic diagram of the SIRT2 3′‐UTR with two putative miR‐212‐5p binding sites, A and B (left), and WT and site A‐ and site B‐mutated SIRT2 3′‐UTRs (right). B, SW480 cells were co‐transfected with miR‐212‐5p and promoter‐driven luciferase reporter constructs expressing WT or mutated SIRT2 3′‐UTR. Luciferase activity was measured. **P < .01 vs the control group; n = 3. N. S., not significant. C, Western blot assay for SIRT2 expression in SW480 and SW620 cells transfected with miR‐212‐5p and anti‐miR‐212‐5p, respectively. β‐actin was used as an internal control. UTR, untranslated region; WT, wild‐type; mut, mutant