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. 2020 Jul 18;24(17):9908–9924. doi: 10.1111/jcmm.15590

Figure 4.

Figure 4

Hsa_circ_0128846 directly targeted miR‐1184. (A) The Circular RNA Interactome database was used to obtain the binding site between hsa_circ_0128846 and miR‐1184. (B) The luciferase reporter assay was used to validate the relationship between hsa_circ_0128846 and miR‐1184 in SW480 and HCT116 cells. *P < .05 versus the miR‐NC subgroup. (C) The hsa_circ_0128846 was proved to be a sponge for miR‐1184 by RIP assay. *P < .05, **P < .001, compared with miR‐1184‐Ago2 group. (D) The expression of miR‐1184 was down‐regulated in tumour tissues. (E) MiR‐1184 expression was negatively correlated with hsa_circ_0128846 expression. (F) qRT‐PCR was employed to determine the expression of hsa_circ_0128846 and miR‐1184 in the SW480 and HCT116 cells with different transfection. Blank, the cells were cultured without any treatments. NC, the cells were transfected with negative control plasmids. sh‐circ, the cells were transfected with circ_0128846 shRNA plasmids. Inhibitor, the cells were transfected with miR‐1184 inhibitor plasmids. sh‐circ + inhibitor, the cells were co‐transfected with circ_0128846 shRNA and miR‐1184 inhibitor plasmids. *P < .05, **P < .001 versus blank group