FIGURE 1.

Cytisine inhibited BMMs and RAW264.7 cells into osteoclast in vitro. A, The chemical structure of Cytisine; B, The cytotoxicity of various concentrations of Cytisine on BMMs and RAW264.7 cells in different time was performed by CCK‐8 assay; C, Representative of TRAP‐positive images of osteoclast from BMMs cells onto different concentrations of Cytisine. Scale bar were 500 μm; D, Representative of TRAP‐positive images of osteoclast from RAW264.7 cells onto different concentrations of Cytisine. Scale bar were 500 μm; (E,F) The quantification of TRAP‐positive multinuclear cells (nuclei > 3 of BMMs and nuclei > 5 of RAW264.7 cells) were shown in C and D. Data are presented with mean ± SD, *P < 0.05; **P < 0.01