FIGURE 4.

Cytisine down‐regulated osteoclastogenesis gene and protein marker expression. A, Real‐time PCR was performed onto RAW264.7 cells to detect Cytisine with or without RANKL that focused on osteoclast‐related gene expression of Cathepsin K, MMP‐9, TRAP and NFATc1. The expression levels of these genes were normalized with GAPDH. B, Western blotting was measured to discover Cytisine with or without RANKL onto RAW264.7 cells that focused on osteoclast‐related protein marker expression of Cathepsin K, CTR, TRAP, MMP‐9 and NFATc1 at different time point. C, Quantification of osteoclast‐related protein expression at different time point according to Western blotting. Data are presented with mean ± SD, *P < 0.05; **P < 0.01; ***P < 0.001