FIGURE 5.

Cytisine inhibited RANKL‐induced activation of MAPK and NF‐κB signalling pathway. A, Representative images of Western blot demonstrating the effect on phosphorylation of JNK, ERK, p38 and total protein induced by RANKL with or without Cytisine at different time point. B, Quantification of osteoclastogenesis‐related protein and JNK/EKR/p38 MAPK pathway protein markers expression levels. C, Representative images of Western blot demonstrating the effect on phosphorylation of IκBα, p65 and total protein induced by RANKL with or without Cytisine at different time point. D, Quantification of osteoclastogenesis‐related protein and IκBα, p65/NF‐κB pathway protein markers expression levels. E, The immunofluorescence staining was performed of location for p65 in the presence or absence of RANKL in RAW 264.7 cells. F, The ratio of fluorescence intensities at nuclear site relative of whole‐cell fluorescence intensity. Data are presented with mean ± SD, *P < 0.05, **P < 0.01