FIGURE 2.

CircRHOT1 is overexpressed and affects the biological function of pancreatic cancer cells. A, Relative expression of circRHOT1 in PDAC cells and human pancreatic ductal cell line cells was measured by RT‐qPCR. B, Relative expression levels of circRHOT1 after transfection of PANC‐1 cells were measured by RT‐qPCR. C, The viability of PANC‐1 cells after transfection was detected by Cell Counting Kit‐8. D, 5‐Ethynyl‐20‐deoxyuridine assays were used to detect cell proliferation after transfection. E, Colony formation assays were used to detect clonogenic ability of PANC‐1 cells after transfection. F, Transwell assays were used to detect cell invasion capacities in PANC‐1 cells after transfection. G, Flow cytometric assays were used to detect apoptosis of PANC‐1 cells after transfection. H, Flow cytometric assays were used to observe the cell cycle after transfection.* P < .05, ** P < .01