FIGURE 5.

E2F3 is involved in modulating the biological function of PANC‐1 cells. A and B, Relative E2F3 expression was measured by RT‐qPCR and Western blot after E2F3 knockdown. C and D, Cell Counting Kit‐8 and 5‐ethynyl‐20‐deoxyuridine assays were used to detect the viability of PANC‐1 cells after transfection. E, Colony formation assays were used to detect colony‐forming capacity in PANC‐1 cells after transfection. F, Transwell assays were used to detect cell invasion capacities in PANC‐1 cells after E2F3 knockdown. G and H, Flow cytometric assays were used to detect apoptosis and to assess the cell cycle after E2F3 knockdown. * P < .05, ** P < .01