FIGURE 2.

Impact of acriflavine (ACF) on the cell cycle of K562 cells. A, K562 cells treated with ACF for 72 h were stained with 7‐AAD and Alexa Fluor‐conjugated anti‐Ki67 or Alexa Fluor‐conjugated IgG isotype control. The distributions of cell cycle phases were then estimated by flow cytometry. One representative experiment is shown (upper part). Data are presented as mean ± SD of three independent experiments (lower part). B, Protein extracts from K562 cells cultured for 72 h with increasing concentrations of ACF were analysed by Western blot with the indicated antibodies (n = 3). Actin served as the loading control